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Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

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Authors: Bao-an Chen, Bin-bin Lai, Jian Cheng, et al

Published Date September 2009 , Volume 2009:4

Journal: International Journal of Nanomedicine

Bao-an Chen1, Bin-bin Lai1, Jian Cheng1, Guo-hua Xia1, Feng Gao1, Wen-lin Xu2, Jia-hua Ding1, Chong Gao1, Xin-chen Sun3, Cui-rong Xu1, Wen-ji Chen1, Ning-na Chen1, Li-jie Liu4, Xiao-mao Li5, Xue-mei Wang6

1Department of Hematology, 3Department of Oncology, the Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People’s Republic of China; 2Department of Hematology, the Affiliated People’s Hospital, Jiangsu University, Zhenjiang, People’s Republic of China; 4Institution of Physiology, 6State Key Lab of Bioelectronics (Chien-shiung Wu Laboratory), Southeast University, Nanjing, People’s Republic of China; 5Department of Physics, University of Saarland, Saarbruechen, Germany

Abstract: Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe3O4, and group D received DNR-loaded MNPs-Fe3O4. For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe3O4 can overcome MDR in vivo.

Keywords: multidrug-resistance reversal, leukemia, magnetic nanoparticles of Fe3O4, in vivo


 

Other articles by Dr Baoan Chen

Effect of Fe3O4-magnetic nanoparticles on acute exercise enhanced KCNQ1 expression in mouse cardiac muscle
Effect of magnetic nanoparticles of Fe3O4 and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells
Reversal of multidrug resistance by magnetic Fe3O4 nanoparticle copolymerizating daunorubicin and 5-bromotetrandrine in xenograft nude-mice
Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
Synergistic effect of the combination of nanoparticulate Fe3O4 and Au with daunomycin on K562/A02 cells
The reversal effect of magnetic Fe3O4 nanoparticles loaded with cisplatin on SKOV3/DDP ovarian carcinoma cells
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